Born in Antakya, Ahmet Payaslı completed his primary, secondary and high school education in Antakya.
He graduated from Gazi University Faculty of Medicine in 1997.
From 1997 to 1998, he worked as a physician at the Ministry of Forestry.
In 1998, he passed the Specialty Examination in Medicine with honors and started his specialty training in Ankara Zekai Tahir Burak Gynecology Training and Research Hospital.
He worked as a gynecologist and obstetrician at Zonguldak Çaycuma State Hospital between 2003-2004.
After working in Ankara Atatürk Training and Research Hospital in 2005-2006, he returned to Ankara Zekai Tahir Burak Gynecology and Obstetrics Hospital in 2006, which he considers to be his professional home. He spent his great time in High Risk Pregnancy Department.
In 2010, he was awarded the ÜYTE (IVF) certificate.
Between 2012 and 2014, he worked as an IVF Responsible at Bahçeci Kosova Hospital.
In 2014, he opened his clinic ''Klinika Dr Payaslı'' in Kosovo. He focused on Laparoscopy, Hysteroscopy, and IVF procedures and performed thousands of operations and IVF procedures. He gave consultancy to many centers about IVF.
In 2019, he returned to Turkey and started to work in Payaslı Clinic.